Zebra Fish Embryo Assay for Toxicological riSk assessment
Chez les poissons, le test Easzy a permis de montrer que de nombreux PE sont capables de perturber, au stade embryonnaire, l’expression d’une enzyme, cytochrome P450 aromatase B, responsable de la synthèse des neuroestrogènes dans les cellules gliales radiaires. Ces cellules jouent un rôle important dans le développement du cerveau puisqu’elles donnent naissance à de nouvelles cellules dont certaines se différencient en neurones. La stratégie du projet FEATS repose sur la conduite d’expérimentations sur des modèles de poissons zèbres exposés à un panel de substances a priori connues pour avoir des effets sur l’expression du cytochrome P450 aromatase B afin d’explorer les effets sur le développement du cerveau et du comportement.
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Analyse préalable à une gestion durable des pêcheries de bouquet en Normandie
Le projet vise à mettre en place une étude ciblée sur le bouquet (Palaemon serratus) par une approche écosystémique des pêches dans un contexte de changement global.
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Gonadal aromatase inhibition and other toxicity pathways leading to Fecundity Inhibition in Zebrafish: from initiating events to population impacts (GinFiz)
The GinFiz project aims to develop and test new methods to characterize the unintended effects of plant protection products on fish populations, particularly the impacts resulting from disruptions to individuals' endocrine systems.The overall goal of this project is the development and improvement of a quantitative Adverse Outcome Pathway (qAOP) leading to decreased fertility, in order to accurately predict the impacts of endocrine disruptors (EDs) across different biological levels — from internal accumulated doses to population-level effects.To achieve this, the project will pursue three main objectives:(i) Enhance the quantitative version of the aromatase A-based AOP, particularly to improve predictions of population-level impacts, using mechanistic mathematical models available at Ineris for zebrafish [18, 20], toxicological data generated by the AIDEZ project, and new data on steroid hormone dynamics.(ii) Integrate into this qAOP an additional toxicity pathway likely involving the progestogen signaling pathway, which may also lead to reduced zebrafish fertility.(iii) Use this qAOP and all generated data in a regulatory context to demonstrate its relevance for implementing the ECHA/EFSA guidance for identifying endocrine-disrupting properties. ...
Interspecies differences in response to endocrine disruptive chemicals
L’objectif du projet INDEE vise à caractériser et identifier des PE ayant une activité endocrinienne spécifique selon l’espèce cible (i.e. humain vs poisson zèbre) dans des effluents urbains et des eaux de rivières par une démarche bio-analytique utilisant une batterie de bioessais in vitro humains et de poisson permettant d’établir des profils d’activités multi-RN et, en combinaison avec des méthodes analytiques, d’identifier, au sein des mélanges, les molécules responsables d’effets inter-espèces.
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Exploring the functional diversity and activity of microbial communities related to mercury cycling in Lake Geneva with new omics approaches
Mercury (chemical symbol Hg) is considered as a priority pollutant, including in Europe, mainly because of the toxicity of its organic form, methylmercury (MeHg), and its propensity to biomagnify, i.e. to increase its concentration in the tissues of organisms as it travels up the food chain. It is known that the Hg methylation, the transformation of divalent Hg (HgII) to MeHg, occurs under oxygen-free conditions and depends essentially on the activity of microorganisms characterized by specific genes – the hgcAB gene cluster. In addition, HgII methylation depends on the HgII bioavailability, the abundance of electron acceptors, the abundance and nature of organic matter as well as the activity and structure of the microbial community. It is therefore instrumental to understand the compendium of metabolic processes that can affect directly or indirectly HgII methylation. This project aims to determine and compare HgII-methylation, microbial biodiversity and activity involved in HgII methylation in contrasted physico-chemical contexts in Lake Geneva. We aim to overcome some of the current limits for predicting MeHg concentrations in the environment.
This project will be a proof of concept of the interest of coupling cutting-edge high-throughput biological analysis (metagenomics and metaproteomics, i.e. global analysis of genes and proteins, respectively) with a physico-chemical characterization. An increased knowledge of the relationship between microbial community activity, physico-chemical conditions, MeHg production and demethylation is necessary to predict the variability in MeHg concentrations across environments and consequently mitigate the Hg methylation to protect environment and human health .
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High-Throughput Multi-Cell Platform for the Analysis of Endocrine Disruptive Stress in Fish
The POMELO project focused on the "stress-on-stress" effects of exposure to tebuconazole (TBZ) and infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss). The study involved three exposure conditions to TBZ: a control (no pollutant), an environmentally relevant concentration (230 ng/L), and an acute/accidental concentration (10 µg/L). Blood and tissue samples (liver, gills, gonads) were collected at multiple time points post-contamination (1, 7, and 28 days; T1–T3). Following 28 days of chemical exposure, surviving trout from each condition were subjected to a viral challenge using the IHNV strain N61 via immersion, or maintained as non-infected controls. Additional sampling was conducted at 48 and 96 hours post-infection (T4–T5).A comprehensive set of analyses was performed, including transcriptomic, epigenetic, immunological, histopathological, and metagenomic assessments. Special attention was given to evaluating impacts on energy metabolism, antioxidant and detoxification pathways, immune parameters, reproductive function, microbiome composition (circulating and mucosal), and DNA methylation patterns. Complementary in vitro assays on human cell lines were also conducted to investigate the impact of TBZ on epigenetic regulators. Finally, biobanking strategies (e.g., FTA cards, tissue microarrays) were implemented to enable future targeted analyses. This integrative, high-throughput multi-omics approach provided a robust framework to investigate endocrine-disrupting stress in fish and supports the development of mechanistically informed environmental risk assessments. ...
Réduction de la pollution des perturbateurs endocriniens à la source : mise à disposition d'outils innovants
Le projet RedPol se concentre sur la question des perturbateurs endocriniens. Les perturbateurs endocriniens sont des substances chimiques qui peuvent interférer avec les systèmes endocriniens (ou hormonaux) à certaines doses et peuvent provoquer des tumeurs cancéreuses, des anomalies congénitales et d'autres troubles du développement chez les êtres humains. Cela a également un impact sur l'environnement au sens large, entraînant des problèmes de reproduction et d'infertilité chez la population d'animaux sauvages, ce qui peut générer un déséquilibre dans la répartition de la population entre les hommes et les femmes à la base de la chaîne alimentaire.
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Surveillance des norovirus humains et des indicateurs de la contamination virale dans un contexte de santé publique : développement d’une approche d’encagement d’invertébrés couplée à de la modélisation
Le projet ANSES 2020-038 SENTINEL propose une approche originale basée sur la biosurveillance active recourant à l’utilisation d’invertébrés accumulateurs de la contamination : Dreissena polymorpha et Gammarus fossarum, couplée à la définition de modèles cinétiques de bioaccumulation. Ce projet a pour ambition de proposer un outil de diagnostic de la contamination virale des masses d’eau via la conversion en termes de charges du milieu aquatique des teneurs en NoV et/ou de ses indicateurs potentiels (FRNAPH) mesurés dans les organismes encagés. Pour atteindre les objectifs, le consortium a associé des compétences et expertises diverses dans le domaine des sciences environnementales (écotoxicologie, UMR-I 02 SEBIO, INRAE ; modélisation : UMR 5558 LBBE et virologie : UMR 7564 LCPME). ...
Monitoring of human noroviruses and indicators of viral contamination in a public health context: development of an invertebrate caging approach coupled with modelling.
The ANSES 2020-038 SENTINEL project proposes an original approach based on active biomonitoring using contamination-accumulating invertebrates: Dreissena polymorpha and Gammarus fossarum, coupled with the definition of kinetic bioaccumulation models. The aim of this project is to propose a tool for diagnosing viral contamination of water bodies by converting NoV levels and/or its potential indicators (FRNAPH) measured in encaged organisms into terms of loads in the aquatic environment. To achieve its objectives, the consortium has brought together a wide range of skills and expertise in the environmental sciences (ecotoxicology, UMR-I 02 SEBIO, INRAE; modelling: UMR 5558 LBBE and virology: UMR 7564 LCPME). ...
